Figure 5: CFP-fluorescence expression of the rat hepatic ASC clone B3 is stable after induction of differentiation. Flow cytometry analysis was performed with cultures of the nontransfected parental rat hepatic ASC strain, Lig-8 (a), and CFP-fluorescent clone B3 hepatic ASC cultures. The B3 cells were evaluated under routine (b) and differentiating (c) culture conditions. Histograms plot the relative numbers of cells as a function of the log-relative CFP fluorescence per cell. The background fluorescence, as defined by nontransfected Lig-8 cells is depicted as the R1 region, and positive fluorescence is denoted by the R2 region. Numbers indicate the percent of cells positive for CFP fluorescence expression.