Table 1: Relative transfection efficiency of fluorescent gene markers and cloning efficiency of selected transfected cell colonies. Transfection efficiency is defined as the average number of colonies/estimated number of cells transfected/μg DNA. Transfections included cells. Cloning efficiency is defined as the number of stable cell strains derived/number of colonies cultured.

Fluorescent marker Average colony number/10-cm dish Relative transfection efficiency Number of clones picked Number of Stable cell strains derived Cloning efficiency

GFP1.3 ( )0.02800%
CFP64 ( )1.0066100%
YFP61 ( )0.9566100%