A Sensitive and Rapid Assay for Investigating Vertical Transmission of Hepatitis B Virus via Male Germ Line Using EGFP Vector as Reporter
Figure 3
Identification of the recombinant plasmid pIRES2-EGFP-HBs. (a) PCR result of
HBs gene before transformation. M: Markers; 1: HBs gene; 2: negative control. (b) PCR
result of HBs gene after transformation. M: Markers; 1 and 2: HBs gene; 3: negative control
after transformation. (c) Result of enzyme digestion. M: Markers; 1 and 2: pRIS2-EGFP-HBs
digested using EcoiR I and Sal I after transformation; 3: pRIS2-EGFP empty as native
control.
