Preparation and Characterization of a Novel Chimeric Protein VEGI-CTT
in Escherichia coli
Figure 3
Expression analysis of recombinant VEGI-CTT in BL21(DE3). (a)
Time course analysis of recombinant VEGI-CTT induction in BL21(DE3)
induced by 1 IPTG. Protein samples were prepared from the
inclusion-body fraction of induced bacterial cells at time points of
2 hours (lane 3), 4 hours (lane 4), 6 hours (lane 5), and 8 hours
(lane 6) of the
induction, and were resolved on 12% SDS-PAGE. Lane 1: low molecular weight
protein markers; lane 2: uninduced control. No difference was found
from lane 3 to lane 6 at 2–8 hours. (b) SDS-PAGE analysis of
expression and location of the fusion protein VEGI-CTT in the
cellular fractions of E. coli. Lane 1: eluted
fraction under renaturing conditions with 500 imidazole; lane 2:
low molecular weight protein markers; lane 3: the inclusion-body
fraction; lane 4: supernatant of cell lysates.
