Research Article
Preparation and Characterization of a Novel Chimeric Protein VEGI-CTT
in Escherichia coli
Table 1
Purification of chimeric protein VEGI-CTT from inclusion
bodies (from 1 L of E. coli culture and 4.6 g wet weight cells).
| | Steps | Total protein (mg) | Step yield (%) | Overall yield (%) |
| | Cell
lysate | 304 | — | — |
| | Inclusion
body | 155 | 100 | 100 |
| | Solubilization
(denaturation buffer containing 8 M urea) | 96.3 | 62.1 | 62.1 |
| | Refolding
and
affinity
purification | 25.5 | 26.6 | 16.5 |
|
|
