Mouse Mammary Tumor Virus Promoter-Containing Retroviral Promoter Conversion Vectors for Gene-Directed Enzyme Prodrug Therapy are Functional in Vitro and in Vivo
Figure 2
In vitro infection of T-47D/DsRed and CRFK/DsRed cells with pPCEMm1. (a)FACS analysis of CRFK/DsRed and T-47D/DsRed cells cocultivated with 2GP19Talf cells stably transfectedwith pPCEMm1 over a time period of five weeks. The numbers of red, green, andred/green fluorescent cells of a representative experiment are shown. As negative controls, 2GP19Talf, T-47D, and CRFK cells were used. Aspositive controls, pure 2GP19Talf/pPCEMm1, T-47D/DsRed, and CRFK/DsRed cellswere used. (b) Confocal laser-scanning microscopy of CRFK/DsRedcells cocultivated with 293/pPCEMm1 cells over a time period of five weeks.Upper left (UL), green fluorescence; upper right (UR), transmission; lower left (LL), redfluorescence; lower right (LR), UL + LL. Magnification 400 x. (c) Confocal laser-scanningmicroscopy of CRFK/DsRed cells cocultivated with 2GP19Talf/pPCEMm1cells over atime period of five weeks. Upper left (UL), green fluorescence; upper right (UR), red fluorescence; lower left (LL), transmission;lower right (LR), UL + UR. Magnification 400 x.
