Mouse Mammary Tumor Virus Promoter-Containing Retroviral Promoter Conversion Vectors for Gene-Directed Enzyme Prodrug Therapy are Functional in Vitro and in Vivo
Figure 3
In vivo infection of T-47D/DsRed and CRFK/DsRed cells with pPCEMm1. (a) FACS analysis of cellsfrom explanted mixed tumors consisting of 2GP19Talf/pPCEMm1cells mixed with either T-47D/DsRed or CRFK/DsRed cells. The numbers of red, green, and red/green fluorescent cells weredetermined. Percentages of red/green doublefluorescent cells for individual tumors are shown. Individualtumors are designated with alphabetical characters below each bar. (b)-(c) Confocal laser-scanning microscopy of cells from explanted mixed tumors consisting of 2GP19Talf/pPCEMm1and either T-47D/DsRed (b) or CRFK/DsRed cells (c). Upper left (UL), green fluorescence; upper right (UR), red fluorescence; lower left (LL), transmission;lower right (LR), UL + UR. Magnification 400 x.
