Mouse Mammary Tumor Virus Promoter-Containing Retroviral Promoter Conversion Vectors for Gene-Directed Enzyme Prodrug Therapy are Functional in Vitro and in Vivo
Figure 5
CYP2B1 expression and activity in T-47D cells stably infected with pPCCMm1. (a)Expression of CYP2B1 in a population of T-47D/pPCCMm1cells. Cell lysates (equal amounts of protein) were analyzed by Westernblotting for the presence of CYP2B1 using an anti-CYP2B1 antibody. The CYP2B1band is indicated with an arrow. The molecular weights of the protein standard are given on the leftside. Neg. cntrl.: T-47D cells not infected with pPCCMm1. (b) Enzymaticactivity of CYP2B1 in a population of T-47D/pPCCMm1 cells. The enzymaticactivity as determined by a resorufin assay is shown as the relative emissionintensity of the created product resorufin at 590 nm in comparison to noninfectedT-47D cells (neg. cntrl.) and a reference clone of virus-packing cells (22P1G).
