The Cyclodextrin Glycosyltransferase of Paenibacillus pabuli US132 Strain: Molecular Characterization and Overproduction of the Recombinant Enzyme
Figure 3
Influence of the operating temperature on the production
of soluble US132 CGTase by E. coli ER2566/pSJ8 strain. Protein samples
loaded onto the 10% SDS-polyacrylamide gel were stained with Coomassie
Brilliant Blue. Lanes 1, 2, and 3: periplasmic fractions obtained from cells
cultivated at 37, 19, and 37–19°C, respectively. Lane M: low molecular weight
marker. Lanes 4, 5 and 6: insoluble fractions obtained when cells are
cultivated at 37–19, 19, and 37°C, respectively.
