The Cyclodextrin Glycosyltransferase of Paenibacillus pabuli US132 Strain: Molecular Characterization and Overproduction of the Recombinant Enzyme
Figure 5
Evolution of bacterial cell growth
and the active US132 CGTase production generated by E. coli ER2566/pSJ8 cultivated in 2TY medium. Induction was performed by 16 mg/L IPTG when
culture reached OD600 nm
between 0.8 and 1. Following the
IPTG induction, the culture temperature is shifted from 37 to 19°C.