The Leishmania HSP20 Is Antigenic during Natural Infections, but, as DNA Vaccine, It does not Protect BALB/c Mice against Experimental L. amazonensis Infection
Figure 2
Phylogenetic analysis of sHSPs. Phylogenetic trees were constructed on the basis of α-crystallin/HSP20 domains by Neighbor Joining (a) and Minimum Evolution (b) using the MEGA 3 program as described in the Material and Methods section. Sequences (UniProtKB/TrEMBL
entry): Afu, Archaeoglobus fulgidus (O28308); Aja, Artibeus jamaicensis (P02482); Aor, Aspergillus oryzae (Q2TXY8); Asf, Aspergillus fumigatus (Q4WV00); Ath, Arabidopsis thaliana (O81822); Avi, Azotobacter vinelandii (P96193); Bap, Buchnera aphidicola (P57640); BjaB, Bradyrhizobium japonicum
(HspB; P70918); BjaF, Bradyrhizobium japonicum (HspF; O69243); Cel,
Caenorhabditis elegans (Q7JP52); Cim, Coccidioides immitis (Q1E6R4); Cma, Caldivirga maquilingensis (A8MB44);
Cpa, Carica papaya (Q69BI7); Ddi, Dictyostelium discoideum (Q54I91); EcoA, Escherichia coli (ibpA; P0C054);
EcoB, Escherichia coli (ibpB; P0C058); Hsa, Homo sapiens (P02489);
Lam, L. amazonensis; Lbr,
L. braziliensis; Lin,
L. infantum; Lma,
L. major; Lpi, Lygodactylus
picturatus (Q6EWI0); Mba,
Methanosarcina barkeri (Q46E59); Mmu, Macaca mulatta (P02488); Mtu, Mycobacterium tuberculosis (P0A5B7); Nfi, Neosartorya fischeri (A1DEG0); Oyp,
Onion yellows phytoplasma (P81958); Pab, Pyrococcus abyssi (Q9V1L0); Pfa, Plasmodium falciparum (Q8IB02); Pis, Pyrobaculum islandicum (A1RRY3); Pgl, Picea glauca (Q40852); Sal, Streptomyces albus (Q53595); Sce, S. cerevisiae (P15992); Sly, Solanum lycopersicum (O82545); Sth,
Streptococcus thermophilus (P80485);
Tbr, Trypanosoma brucei (Q57V53); Tgo, Toxoplasma gondii (Q6DUA8);
Tsc, Trachemys scripta (Q91517); Zma, Zea mays (P24632). The scale represents mutational changes per residue.
