Expression and Hydroxylamine Cleavage of Thymosin Alpha 1 Concatemer
Figure 4
Overexpression of the recombinant concatemer protein. (a) Lane 1: prestained
molecular mass markers. Lanes 2 and 3: full bacterial protein from 100 L
transformed cells after 1.0 mM and 0.5 mM IPTG induction
for 8 hours at 37°C. Lane 4: full bacterial protein from 100 L transformed cells without induction
of IPTG for 8 hours at 37°C. (b) Lane 1: prestained molecular mass
markers. Lane 2: full bacterial protein from 100 L transformed cells after 1.0 mM IPTG induction for 8 hours. Lane
3: sedimented material from Sonication
of 100 L transformed cells after induction with IPTG. Lane 4: suspended material
from Sonication of 100 L transformed cells after induction with IPTG.
