BioMed Research International / 2008 / Article / Fig 4

Research Article

Expression and Hydroxylamine Cleavage of Thymosin Alpha 1 Concatemer

Figure 4

Overexpression of the recombinant concatemer protein. (a) Lane 1: prestained molecular mass markers. Lanes 2 and 3: full bacterial protein from 100  L transformed cells after 1.0 mM and 0.5 mM IPTG induction for 8 hours at 37°C. Lane 4: full bacterial protein from 100  L transformed cells without induction of IPTG for 8 hours at 37°C. (b) Lane 1: prestained molecular mass markers. Lane 2: full bacterial protein from 100  L transformed cells after 1.0 mM IPTG induction for 8 hours. Lane 3: sedimented material from Sonication of 100  L transformed cells after induction with IPTG. Lane 4: suspended material from Sonication of 100  L transformed cells after induction with IPTG.
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736060.fig.004b
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