Resistance of FANCA-mutant cells with defective FANCD2 function to hydrogen peroxide (H2O2).
(a) Representative images illustrating staining for subnuclear FANCD2 foci in isogenic fibroblast pairs, either deficient for FANCD2 (PD20) or FANCA (PD220) and their respective wild-type (wt) complemented counterparts. Foci were visualized three hours after treatment with
H2O2 (25 μM for 2 hours). (b) Clonogenic survival of cells with or without wild-type FANCA after treatment with varying concentrations of H2O2. Data
represent logarithmic means +/− standard error from 15-repeat experiments.
