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Journal of Biomedicine and Biotechnology
Volume 2009, Article ID 126986, 9 pages
http://dx.doi.org/10.1155/2009/126986
Research Article

Cloning, Identification, and Characterization of the rpoS-Like Sigma Factor rpoX from Vibrio alginolyticus

1Key Laboratory of Marine Bio-resources Sustainable Utilization (LMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences (CAS), Guangzhou 510301, China
2Graduate University of the Chinese Academy of Sciences, Beijing 100039, China

Received 26 March 2009; Revised 5 August 2009; Accepted 28 September 2009

Academic Editor: Roy Gross

Copyright © 2009 Jing-jing Zhao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. S. Borukhov and E. Nudler, “RNA polymerase holoenzyme: structure, function and biological implications,” Current Opinion in Microbiology, vol. 6, no. 2, pp. 93–100, 2003. View at Publisher · View at Google Scholar · View at Scopus
  2. R. Hengge-Aronis, “A role for the σS subunit of RNA polymerase in the regulation of bacterial virulence,” Advances in Experimental Medicine and Biology, vol. 485, pp. 85–93, 2000. View at Google Scholar
  3. M. S. B. Paget and J. D. Helmann, “The σ70 family of sigma factors,” Genome Biology, vol. 4, no. 1, p. 203, 2003. View at Publisher · View at Google Scholar · View at Scopus
  4. M. Lonetto, M. Gribskov, and C. A. Gross, “The σ70 family: sequence conservation and evolutionary relationships,” Journal of Bacteriology, vol. 174, no. 12, pp. 3843–3849, 1992. View at Google Scholar · View at Scopus
  5. J. D. Helmann, “The extracytoplasmic function (ECF) sigma factors,” Advances in Microbial Physiology, vol. 46, pp. 47–110, 2002. View at Google Scholar
  6. R. Lange and R. Hengge-Aronis, “Identification of a central regulator of stationary-phase gene expression in Escherichia coli,” Molecular Microbiology, vol. 5, no. 1, pp. 49–59, 1991. View at Publisher · View at Google Scholar · View at Scopus
  7. F. C. Fang, S. J. Libby, N. A. Buchmeier et al., “The alternative s factor katF (rpoS) regulates Salmonella virulence,” Proceedings of the National Academy of Sciences of the United States of America, vol. 89, no. 24, pp. 11978–11982, 1992. View at Publisher · View at Google Scholar · View at Scopus
  8. J. L. Badger and V. L. Miller, “Role of RpoS in survival of Yersinia enterocolitica to a variety of environmental stresses,” Journal of Bacteriology, vol. 177, no. 18, pp. 5370–5373, 1995. View at Google Scholar · View at Scopus
  9. F. H. Yildiz and G. K. Schoolnik, “Role of rpoS in stress survival and virulence of Vibrio cholerae,” Journal of Bacteriology, vol. 180, no. 4, pp. 773–784, 1998. View at Google Scholar · View at Scopus
  10. M. I. Ramos-Gonzalez and S. Molin, “Cloning, sequencing, and phenotypic characterization of the rpoS gene from Pseudomonas putida KT2440,” Journal of Bacteriology, vol. 180, no. 13, pp. 3421–3431, 1998. View at Google Scholar · View at Scopus
  11. S. J. Suh, L. Silo-Suh, D. E. Woods, D. J. Hassett, S. E. H. West, and D. E. Ohman, “Effect of rpoS mutation on the stress response and expression of virulence factors in Pseudomonas aeruginosa,” Journal of Bacteriology, vol. 181, no. 13, pp. 3890–3897, 1999. View at Google Scholar · View at Scopus
  12. Y. H. Lin, C. Miyamoto, and E. A. Meighen, “Cloning, sequencing, and functional studies of the rpoS gene from Vibrio harveyi,” Biochemical and Biophysical Research Communications, vol. 293, no. 1, pp. 456–462, 2002. View at Publisher · View at Google Scholar · View at Scopus
  13. T. M. Rosche, D. J. Smith, E. E. Parker, and J. D. Oliver, “RpoS involvement and requirement for exogenous nutrient for osmotically induced cross protection in Vibrio vulnificus,” FEMS Microbiology Ecology, vol. 53, no. 3, pp. 455–462, 2005. View at Publisher · View at Google Scholar · View at Scopus
  14. B. Weber, A. Croxatto, C. Chen, and D. L. Milton, “RpoS induces expression of the Vibrio anguillarum quorum-sensing regulator VanT,” Microbiology, vol. 154, no. 3, pp. 767–780, 2008. View at Publisher · View at Google Scholar · View at Scopus
  15. Y. Tian, Q. Y. Wang, Q. Liu, Y. Ma, X. D. Cao, and Y. Zhang, “Role of RpoS in stress survival, synthesis of extracellular autoinducer 2, and virulence in Vibrio alginolyticus,” Archives of Microbiology, vol. 190, no. 5, pp. 585–594, 2008. View at Publisher · View at Google Scholar · View at Scopus
  16. M. C. Balebona, M. J. Andreu, M. A. Bordas, I. Zorilla, M. A. Morinigo, and J. J. Borrego, “Pathogenicity of Vibrio alginolyticus for cultured gilt-head sea bream (Sparus aurata L.),” Applied and Environmental Microbiology, vol. 64, no. 11, pp. 4269–4275, 1998. View at Google Scholar · View at Scopus
  17. Z. Y. Xie, C. Q. Hu, C. Chen, L. P. Zhang, and C. H. Ren, “Investigation of seven Vibrio virulence genes among Vibrio alginolyticus and Vibrio parahaemolyticus strains from the coastal mariculture systems in Guangdong, China,” Letters in Applied Microbiology, vol. 41, no. 2, pp. 202–207, 2005. View at Publisher · View at Google Scholar · View at Scopus
  18. C. Chen, J. Xie, and C. Q. Hu, “Phenotypic and genetic differences between opaque and translucent colonies of Vibrio alginolyticus,” Biofouling, vol. 25, no. 6, pp. 525–531, 2009. View at Google Scholar · View at Scopus
  19. V. L. Miller and J. J. Mekalanos, “A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR,” Journal of Bacteriology, vol. 170, no. 6, pp. 2575–2583, 1988. View at Google Scholar · View at Scopus
  20. R. Simon, U. Priefer, and A. Pühler, “A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram negative bacteria,” Bio-Technology, vol. 1, no. 9, pp. 787–796, 1983. View at Google Scholar · View at Scopus
  21. D. L. Milton, R. O'Toole, P. Hörstedt, and H. Wolf-Watz, “Flagellin A is essential for the virulence of Vibrio anguillarum,” Journal of Bacteriology, vol. 178, no. 5, pp. 1310–1319, 1996. View at Google Scholar · View at Scopus
  22. S. Passey, S. Pellegrin, and H. Mellor, “Scanning electron microscopy of cell surface morphology,” Current Protocols in Cell Biology, vol. 37, chapter 4, unit 4.17, pp. 1–13, 2007. View at Google Scholar · View at Scopus
  23. E. A. Campbell, O. Muzzin, M. Chlenov et al., “Structure of the bacterial RNA polymerase promoter specificity s subunit,” Molecular Cell, vol. 9, no. 3, pp. 527–539, 2002. View at Publisher · View at Google Scholar · View at Scopus
  24. A. Ito, T. May, K. Kawata, and S. Okabe, “Significance of rpoS during maturation of Escherichia coli biofilms,” Biotechnology and Bioengineering, vol. 99, no. 6, pp. 1462–1471, 2008. View at Publisher · View at Google Scholar · View at Scopus
  25. J. L. Adams and R. J. C. McLean, “Impact of rpoS deletion on Escherichia coli biofilms,” Applied and Environmental Microbiology, vol. 65, no. 9, pp. 4285–4287, 1999. View at Google Scholar · View at Scopus
  26. M. Anderson, C. E. Pollitt, I. S. Roberts, and J. A. Eastgate, “Identification and characterization of the Erwinia amylovora rpoS gene: rpoS is not involved in induction of fireblight disease symptoms,” Journal of Bacteriology, vol. 180, no. 24, pp. 6789–6792, 1998. View at Google Scholar · View at Scopus
  27. M. J. Mandel, E. V. Stabb, and E. G. Ruby, “Comparative genomics-based investigation of resequencing targets in Vibrio fischeri: focus on point miscalls and artefactual expansions,” BMC Genomics, vol. 9, article 138, 2008. View at Publisher · View at Google Scholar · View at Scopus