BioMed Research International / 2009 / Article / Tab 1

Research Article

Cloning, Identification, and Characterization of the rpoS-Like Sigma Factor rpoX from Vibrio alginolyticus

Table 1

Bacterial strains and plasmids used in this study.

Strain or plasmidRelevant characteristics Reference or source

E. coli
 SY327 (lac pro) argE(Am) rif malA recA56 pir lysogen[19]
 S17-1thi pro hsdR hsdM+ recA RP4-2-Tc::Mu-Km::Tn7 pir lysogen[20]
V. alginolyticus
 ZJ-OOpaque variant of wild strain, isolated from diseased cultured fish in Guangdong province on the Southern China coast. [17]
 ZJ-TTranslucent variant of wild strain; isolated from diseased cultured fish in Guangdong province on the Southern China coast. [17]
rpoX-OZJ-O carrying an in-frame deletion of rpoX. This study
rpoX-TZJ-T carrying an in-frame deletion of rpoX. This study
 CrpoX-O rpoX-O complemented in trans with wild-type rpoX gene. and This study
 CrpoX-T rpoX-T complemented in trans with wild-type rpoX gene. and This study
Plasmids
 pDM4 , suicide vector with an R6K origin(pir requiring) and sacBR of Bacillus subtilis [21]
 pDMrpoX , pDM4 containing a mutant allele of rpoX This study
 pDMCrpoX , pDM4 containing the wild-type allele of rpoX This study

, chloramphenicol resistant
, ampicillin resistant.

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