Experimental Study of the Effects of Marrow Mesenchymal Stem Cells Transfected with Hypoxia-Inducible Factor-1𝛼 Gene

Figure 2

(a) The results of HIF-1𝛼-T vector double digestion with EcoR I and Sal I. 1–3: Clones 1–3; m:𝜆DNA/Hind III; M:DNA marker; Clones 1 and 2 were positive clone. Red arrow showed the HIF-1𝛼 band, and yellow arrow showed the T vector band. (b) The results of HIF-1𝛼-pcDNA_3.1 vector double digestion with Hind III and BamH I. 2-1: HIF-1𝛼-pCDNA_3.1 positive clone; 2-2: pcDNA_3.1 empty vector; M: DL 2000. Red arrow showed the HIF-1𝛼 band, and black arrow showed the pCDNA_3.1 vector band.

(a)

(b)