Experimental Study of the Effects of Marrow Mesenchymal Stem Cells Transfected with Hypoxia-Inducible Factor-1 Gene
(a) The results of HIF-1-T vector double digestion with EcoR I and Sal I. 1–3: Clones 1–3; m:DNA/Hind III; M:DNA marker; Clones 1 and 2 were positive clone. Red arrow showed the HIF-1 band, and yellow arrow showed the T vector band. (b) The results of HIF-1-pcDNA3.1 vector double digestion with Hind III and BamH I. 2-1: HIF-1-pCDNA3.1 positive clone; 2-2: pcDNA3.1 empty vector; M: DL 2000. Red arrow showed the HIF-1 band, and black arrow showed the pCDNA3.1 vector band.
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