Experimental Study of the Effects of Marrow Mesenchymal Stem Cells Transfected with Hypoxia-Inducible Factor-1 Gene
Morphology of MSCs in primary culture (100×). The primary cultured cells floated in medium and had a uniformed globular shape (a). 3-4 days later, the cells adhered to the bottle began division growth (b) and the proliferated cell had a long fusiform shape containing 1–3 nucleolus 5-6 days later (c). The cell colony can be seen 7–9 days later (d) and contact inhibition can be observed 10–12 days later (e). The 3rd generation MSCs was well-distributed as fibroblast.
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