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Journal of Biomedicine and Biotechnology
Volume 2009, Article ID 157531, 8 pages
http://dx.doi.org/10.1155/2009/157531
Research Article

Isolation of Osteosarcoma-Associated Human Antibodies from a Combinatorial Fab Phage Display Library

1Institut Gustave Roussy, UPRES EA 3535 PR2. 39, Rue Camille Desmoulins, 94805 Villejuif, France
2Departamento de Bioquímica e Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, ICB II-Campus II Samambaia, 74001-970 Goiania, GO, Brazil
3Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília, Campus Universitário Darcy Ribeiro, Asa Norte, 70910900 Brasilia, DF, Brazil
4Laboratório de Imunologia Molecular, Universidade de Brasília, Campus Universitário Darcy Ribeiro, Sala ASS 128, ICC Sul, Asa Norte, 70910-900 Brasilia, DF, Brazil

Received 8 February 2009; Revised 29 June 2009; Accepted 9 September 2009

Academic Editor: Anthony L. DeVico

Copyright © 2009 Carmela Dantas-Barbosa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Osteosarcoma, a highly malignant disease, is the most common primary bone tumor and is frequently found in children and adolescents. In order to isolate antibodies against osteosarcoma antigens, a combinatorial osteosarcoma Fab library displayed on the surface of phages was used. After three rounds of selection on the surface of tumor cells, several osteosarcoma-reactive Fabs were detected. From these Fabs, five were better characterized, and despite having differences in their VH (heavy chain variable domain) and (kappa chain variable domain) regions, they all bound to a protein with the same molecular mass. Further analysis by cell ELISA and immunocytochemistry suggested that the Fabs recognize a membrane-associated tumor antigen expressed in higher amounts in neoplasic cells than in normal tissue. These results suggest that the human Fabs selected in this work are a valuable tool for the study of this neoplasia.