Isolation of Osteosarcoma-Associated Human Antibodies from a Combinatorial Fab Phage Display Library

Table 1

Reactivity of randomly selected Fabs toward tumor antigens in ELISA assays.

Cycle of selection(System)	Clones assayed	Positive clones in Cell ELISA^a	Positive clones on Total Protein Extracts^b
2nd (N)	40	10	28
3rd (N)	39	NT^c	13
2nd (O)	48	0	1
3rd (O)	40	1	4

^aA clone was considered positive if the absorbance at 405 nm was three times greater than the absorbance found for hFOB 1.19 cell surface binding.
^bA clone was considered positive if the absorbance at 405 nm was ten times greater than the absorbance observed for empty vector supernatant.
^cNT = Not tested.