p,p^′-DDE Induces Apoptosis of Rat Sertoli Cells via a FasL-Dependent Pathway

Figure 1

Effect of p,p^′-DDE on the viability of rat Sertoli cells with MTT assay. MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) can be reduced by the mitochondrial dehydrogenase of viable cells to blue formazan product which can be measured spectrophotometrically. Cells were treated with 0.3% DMSO, 10, 30, 50 or 70 μM p,p^′-DDE for 24 hours, and the optical density (OD) was measured by MTT. Cellular viability (%) was calculated using the following equation: cellular viability (%)=(OD_treatment/OD_control) × 100. Significant difference is (*) 𝑃<.05, compared with the control group.