p,p^′-DDE Induces Apoptosis of Rat Sertoli Cells via a FasL-Dependent Pathway

Figure 5

(a) Effect of NAC on p,p^′-DDE-induced FasL protein. Sertoli cells were treated with p,p^′-DDE pretreated with or without NAC. Protein from whole cell lysates was used in Western blotting for FasL detection. (b)–(c) Procaspase-3 and -8 in Sertoli cells exposed to p,p^′-DDE for 24 hours. Protein from whole cell lysates was used in Western blotting for procaspase-3 and -8 detection. (d)–(e) Quantitative analysis of the immunoreactive FasL and procaspase-3 and -8. Data are indicated as mean ± SD. Significant difference is (*) 𝑃<.05, compared with the control group is (^#) 𝑃<.05, compared with 50 μM p,p^′-DDE group.

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