Meso Scale Discovery and Luminex Comparative Analysis of Calbindin D28K

Table 1

Linearity analysis of rat cortical kidney homgenate dilutions.

	MSD			Luminex
Dilution factor	Measured pg/mL	CV%^+	% Mean^∗	Measured pg/mL	CV%^+	% Mean^∗
100	5554	1.2	131	49762	0.1	128
200	2410	1.9	114	22802	5.1	117
400	1233	11	116	11568	1.2	119
800	424	12	80	5421	8.0	112
1600	247	6.7	93	2709	5.2	111
3200	110	16	83	1120	3.7	92
6400	55	14	83	422	12	69
12800	—			155	6.2	51

∗Calculated concentration (dilution factor × measured concentration) as a percentage of the average of the calculated concentration from all dilutions. The average calculated concentration of rat cortical kidney homogenate analyzed by MSD and Luminex was 424 ng/mL and 3.9 𝜇g/mL, respectively.+Coefficient of variation.