Low-Frequency Low-Intensity Ultrasounds Do Not Influence the Survival and Immune Functions of Cultured Keratinocytes and Dendritic Cells
Figure 1
US at 42 KHz and 0.15 do not influence ERK1/2 levels in keratinocytes. Keratinocyte cultures treated with 1–3 cycles of US or left untreated or activated with IFN- and TNF- were analyzed by Western blotting analysis performed with an Ab specific for both p42 and p44 ERK phosphorylated at Tyr 204 residue (phospho-ERK1/2). Filters were stripped and reprobed with an anti-ERK1/2 Ab. Equal loading of the samples was assayed probing the filters using an Ab against the -actin protein.
