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Journal of Biomedicine and Biotechnology
Volume 2009 (2009), Article ID 201075, 7 pages
http://dx.doi.org/10.1155/2009/201075
Research Article

Engineering and Directed Evolution of a Ca2+ Binding Site A-Deficient AprE Mutant Reveal an Essential Contribution of the Loop Leu75–Leu82 to Enzyme Activity

1Departamento de Biología, Universidad de Guanajuato, Colonia Noria Alta S/N, Guanajuato, 36050 Guanajuato, Mexico
2Centro de Investigación en Alimentos y Nutrición, Facultad de Medicina, Universidad Juárez del Estado de Durango, Avenida Universidad y Anitúa S/N, 34000 Durango, Mexico
3Departamento de Ciencias Naturales, Universidad Autónoma Metropolitana Unidad Cuajimalpa, Pedro Antonio de los Santos 84, San Miguel Chapultepec 11850, Mexico

Received 12 February 2009; Revised 17 May 2009; Accepted 15 June 2009

Academic Editor: George Makhatadze

Copyright © 2009 Eliel R. Romero-García et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

How to Cite this Article

Eliel R. Romero-García, Alfredo Téllez-Valencia, María F. Trujillo, et al., “Engineering and Directed Evolution of a Ca2+ Binding Site A-Deficient AprE Mutant Reveal an Essential Contribution of the Loop Leu75–Leu82 to Enzyme Activity,” Journal of Biomedicine and Biotechnology, vol. 2009, Article ID 201075, 7 pages, 2009. doi:10.1155/2009/201075