Caenorhabditis elegans F09E10.3 Encodes a Putative 3-Oxoacyl-Thioester Reductase of Mitochondrial Type 2 Fatty Acid Synthase FASII that Is Functional in Yeast

Table 1

Plasmids and oligonucleotides used.

Plasmids or oligonucleotides	Description	Source/reference
Plasmid^a
(1) pBluescript KS II (+)	pKS cloning vector	Stratagene
pKS:F09E10.3^1	F09E10.3 in pBluescript	This study
pKS:D1054.8^1	D1054.8 in pBluescript	This study
(2) YEp352	URA3-marked multicopy plasmid	[3]
(3) YEp:CTA1^2	CTA1 behind its own promoter	[4]
YEp:F09E10.3^3	F09E10.3 behind the CTA1 promoter	This study
YEp:D1054.8^3	D1054.8 behind the CTA1 promoter	This study
YEp:OAR1^3	OAR1 behind the CTA1 promoter	Z.-J. Chen
oligonucleotide^b
Ce OAR1-1 5^′ XbaI	TTATTCTAGATGAGTGCTTTGCTGGGAGG	This study
Ce OAR1-1 3^′ XhoI	TATTCTCGAGCTACATACTAAATCCACCGG	This study
Ce OAR1-2 5^′ XbaI	TTATTCTAGATGACTCGTTTTGCTGAAAAAGTC	This study
Ce OAR1-2 3^′ XhoI	TATTCTCGAGTTAATGTAAAAGTTTTGCAAAG	This study

^aThe superscripts following the plasmid designations refer to their parental vectors, for example, pKS:F09E10.3¹ was derived from (1) pBluescript KS II (+). ^bOligonucleotides Ce OAR1-1 refer to F09E10.3 and Ce OAR1-2 to D1054.8.