Construction of a Recombinant Eukaryotic Expression Plasmid Containing Human Calcitonin Gene and Its Expression in NIH3T3 Cells
Construction and identification of recombinant pCDNA3.0-Ig -hCT eukaryotic expression vector. (a), The sequences that need to be synthesized. The recognition sequences for HindIII, BamHI and XbaI restriction enzymes are shown underline. The stop codon is shown asterisk under it. The DNA sequence of hCT is shown in boldface and italics, and the translated amino acids are under it. (b), Construction procedures of the recombinant pCDNA3.0-Ig-hCT eukaryotic expression vector. (c), Electrophoresis of recombinant plasmid pCDNA3.0-Ig-hCT digested with HindIII and XbaI.
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