Gene Expression Divergence and Evolutionary Analysis of the Drosomycin Gene Family in Drosophila melanogaster
Figure 1
The transcription activity of drosomycin gene family in D. melanogaster stimulated by microbe-free injury and various microorganisms. Quantitative real time RT-PCR was performed for the detection of the expression levels of drosomycin genes in wild-type Oregon-R flies with SYBR Green. The expression levels of drosomycin genes were normalized to rp49 in the samples. The experiments were done in triplicate and the error bars represented standard deviation. CK: native flies. (a) The 2-day-old adult flies were pricked by a sterile needle (microbe-free injury). (b) to (e) The 2-day-old adult flies were infected with E. coli K12D31, Staphylococcus aureus, Fusarium oxysporum, and Beauveria bassiana, respectively.
