BioMed Research International / 2009 / Article / Fig 2

Research Article

Chlamydia trachomatis Alters Iron-Regulatory Protein-1 Binding Capacity and Modulates Cellular Iron Homeostasis in HeLa-229 Cells

Figure 2

Effect of CT infection on IRP-1 expression in HeLa cells: 40  g cell lysates were electrophoresed on 8% SDS-PAGE gels, followed by Western blotting using anti-IRP-1 and anti-IRP-2 antibodies, with beta-actin as control. (a) CT-infected HeLa cells showing higher expression of IRP-1, which declined after addition of DFX. (b) Decline in the level of IRP-2 expression was observed in CT-infected HeLa cells, and there was further decline consequent to addition of DFX. No significant change was observed in FAC-treated control and infected cells. Bars represent standard error. * and *** .

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