Induction of Tetraploids from Petiole Explants through Colchicine Treatments in Echinacea purpurea L.

Table 3

Comparison of the effect of duration of 120 mg/L colchicine treatment on shoot regeneration from petiole explants. Data were collected 40 days after transfer of the colchicine treated explants to colchicine-free regeneration medium.

Treatment duration (days)	% shoot regeneration	No. shoots per bottle
7	72.5 a*	7.0 a
14	60.0 b	5.2 b
21	47.0 c	3.8 c
28	17.5 d	2.2 d

*Data in the same column followed by different letters are significantly different by Duncan’t test at ≤5% level.