Modular Cre/lox System and Genetic Therapeutics for Colorectal Cancer

Figure 5

Negative downregulation using the modular Cre/lox system. Floxed effectors can include negative repressors of the transcriptional activity driving Cre expression. For example, in this model system, DN-Tcf4, which inhibits Wnt activity, is expressed from a floxed vector in a Wnt activity-specific manner. The DN-Tcf4 feeds back to repress Wnt activity in a Wnt activity-specific manner. Generalized systems of this nature can be applied to other disorders in which constitutive high activation of cell signaling causes disease, adapted from [33].