Herpesvirus-Mediated Delivery of a Genetically Encoded Fluorescent Sensor to Canine Cardiomyocytes
Figure 1
This panel shows a part of recombinant herpesvirus genome. To adapt for applicability as virus vector we deleted the two ribonucleotide reductase (rr) and the early protein (ep0) genes from the virus genome by homologue recombination. The rr1 and rr2 genes are in charge for production of raw material of viral DNA. The recombinant virus is not able to productively infect postmitotic cells without rr genes, while ep0 gene is responsible for virus reactivation from latency. Troponeon calcium sensor gene was inserted into the antisense promoter region in two copies.
