Clinical Significance of Epigenetic Inactivation of hMLH1 and BRCA1 in Tunisian Patients with Invasive Breast Carcinoma

Table 1

Summary of primer sequences, annealing temperature, product size and number of cycle used in MSP assay. M: methylated DNA, U: unmethylated DNA, F: forward, R: Reverse.

Gene		Sequence (5^′-3^′)	𝑇𝑚 (°C)	Size (bp)	PCR cycles
hMLH1 (U)	F	TTTTGATGTAGATGTTTTATTAGGGTTGT	60	114	30
	R	ACCACCTCATCATAACTACCCACA
hMLH1 (M)	F	ACGTAGACGTTTTATTAGGGTCGC	58	110	30
	R	CCTCATCGTAACTACCCGCG
BRCA1I   (U)	F	GGTTAATTTAGAGTTTTGAGAGATG	60	75	30
	R	TCAACAAACTCACACCACACAATCA
BRCA1  (M)	F	GGTTAATTTAGAGTTTCGAGAGACG	60	86	30
	R	TCAACGAACTCACGCCGCGCAATCG