BioMed Research International / 2009 / Article / Fig 3

Methodology Report

Automation of cDNA Synthesis and Labelling Improves Reproducibility

Figure 3

Automation of cDNA synthesis and labelling produces a higher correlation between technical replicates. (a) Dendrogram and heatmap of Spearman’s rank correlation between manually labelled (gold cluster, KTH_Man) and automatically labelled (blue cluster, KTH_Auto1, and KTH_Auto2) samples. (b) Boxplot of the well-to-well reproducibility within experiments. Automatically labelled samples (blue boxes) are more highly correlated than manually labelled samples (gold box). Our automated approach also exhibited a higher correlation than between NMC and OPN experiments, but not higher than between the two NCI experiments.

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