Adding to Yersinia enterocolitica Gene Pool Diversity: Two Cryptic Plasmids from a Biotype 1A Isolate
Figure 3
ORF7 of plasmid pYe4449-2 encodes a putative new repeat protein with a high content of phosphorylatable amino acids. (a) Scheme of the arrangement of the ORF7-encoded hypothetical protein. The nonameric repeat module “RTDSLGNTY” occurring twice in each repeat is indicated by dark grey boxes. (b) Alignment of the protein repeat segments encoded by ORF7, residues identical in all three repeats are highlighted against a dark grey background and residues identical in two of the three repeats are highlighted against a light grey background. The repeat module “RTDSLGNTY” occurring twice in each repeat is indicated by a bar below the consensus sequence. Below these bars, the interspecies repeat motif “DxxGNDxxGN” is given as deduced from the closest homologues of ORF 7 (BACOVA_04330; NE2449; MPG2080_02246). Serine and threonine residues most likely to be phosphorylated in a eukaryotic background according to NetPhos 2.0, server predictions (score 0.968 and above) were indicated by circles.
