Adding to Yersinia enterocolitica Gene Pool Diversity: Two Cryptic Plasmids from a Biotype 1A Isolate
Figure 4
Detection of epitope-tagged ORF3 expressed at 4°C. A transposon mutant derivative of isolate 07-04449 carrying plasmid pYe4449-2/ORF3::Tn5 was transformed with plasmid pACYC184/ORF3-RGS(H)6 and pACYC184, respectively, and cultured over night at 4°C in LB broth. Bacterial pellet fractions “P” and TCA-precipitated supernatant fractions “S” were subjected to denaturing SDS-PAGE. Subsequently, the gel was Western-blotted followed by chromogenic detection using a monoclonal antibody directed against the RGS(H)4 epitope. The arrow indicates the ORF3-RGS(H)6 product.
