Mesothelioma Cells Escape Heat Stress by Upregulating Hsp40/Hsp70 Expression via Mitogen-Activated Protein Kinases
Figure 2
Heat stress activates phosphorylation of Erk1/2 and p38 MAPK. (a) Representative immunoblot of p38 MAPK phosphorylation and nuclear accumulation by heat stress (40°C, 20 minutes) in mesothelial cells. Similar results were obtained in two additional experiments. Coomasie blue was used to control equal protein loading (loading control). (b) representative immunoblot of constitutive p38 MAPK phosphorylation in PDMM cells (LO68). Similar results were obtained in the other two PDMM cell lines. (c) representative immunoblot of Erk1/2 phosphorylation and nuclear accumulation after heat stress (40°C, 20 minutes) in PDMM cells (LO68). Similar results were obtained with two other PDMM cell lines and in mesothelial cells.
