Effect of Oxysterol-Induced Apoptosis of Vascular Smooth Muscle Cells on Experimental Hypercholesterolemia

Figure 2

Representative Western blots showing expression levels of Bax, Bcl-xl, and Bcl-2 on SMC cultures at baseline (SMC-C, SMC-Ch, and SMC-Ch-FO) and changes in protein expression after treatment of SMC cultures with 20 𝜇g/mL 25-hydroxycholesterol for 24 hours. Equal amounts of proteins were separated by electrophoresis and processed for immunoblotting. Similar results were obtained in three separate experiments. The ratios were calculated by using O.D protein band reading and normalized against a control (SMC-C) for each protein.