Effect of Oxysterol-Induced Apoptosis of Vascular Smooth Muscle Cells on Experimental Hypercholesterolemia
Figure 4
bcl-xl mRNA quantification in SMC cultures (SMC-C, SMC-Ch, SMC-Ch-FO) and SMC cultures treated with 20 g/mL 25-hydroxycholesterol for 24 hours. mRNA levels were quantified by real-time semiquantitative reverse-transcription PCR. Results are shown as relative expression ratio of bcl-2xl in SMC cultures with respect to control culture and expressed in comparison to reference gene -actin. Bars represent the means SEM for 3 different experiments. *, **, *** versus SMC-C, +, ++, +++ versus SMC-C treated with 20 g/mL 25-hydroxycholesterol.
