Integrative Gene Cloning and Expression System for Streptomyces sp. US 24 and Streptomyces sp. TN 58 Bioactive Molecule Producing Strains
(a) Southern blot of Streptomyces sp. US24 BamHI-digested total DNA hybridised with 32P-labeled pSET152 plasmid. Lane 1, untransformed wild type Streptomyces sp. US24; lanes 2–9, the eight studied exconjugants; lane 10, BamHI-digested pSET152 plasmid DNA (5.7 Kb); lane 11, the 1 kb ladder used as DNA marker. (b) Schematic representation of tandemly insertion of the pSET152 into the Streptomyces sp. US24 attB site. Chromosomal DNA hybridising is represented by thin lines and pSET152 by thick line. The integrase gene (int), and the apramycin resistance gene [aac3(IV)] from pSET152 attP, attL, and attR sites are also shown.
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