Inhibitory Effect of Flavonoids on the Efflux of 𝑁-Acetyl 5-Aminosalicylic Acid Intracellularly Formed in Caco-2 Cells

Table 1

The effect of flavonols and transporter inhibitors on the apical and basolateral efflux of N-acetyl 5-aminosalicylic acid in Caco-2 cells. Caco-2 cells grown in Transwells were incubated with 200 𝜇M 5-ASA for 1, 2, and 4 hours in the presence of flavonols and transporter inhibitors at the concentration of 100 𝜇M. Api: apical efflux of 5-AcASA, Baso: basolateral efflux of 5-AcASA. Each value represents the mean ± SD of four to five experiments.

		Control	Fisetin	Quercetin	Morin	MK571	Quinidine
1hr	Api (nmol)	1.01±0.14	0.16±0.06^**	0.32±0.07^**	0.87±0.03	0.47±0.13^**	0.91±0.16
	Baso (nmol)	0.19±0.02	0.31±0.09^**	0.47±0.08^**	0.43±0.01^**	0.45±0.04^**	0.18±0.03
	Api/Baso	5.32±0.38	0.52±0.11^**	0.68±0.06^**	2.02±0.04^**	1.04±0.19^**	5.06±0.21
2hr	Api (nmol)	2.05±0.28	0.36±0.13^**	0.80±0.21^**	1.64±0.15	1.05±0.05^**	1.96±0.59
	Baso (nmol)	0.29±0.04	0.47±0.11^**	0.94±0.07^**	0.66±0.06^**	0.70±0.03^**	0.28±0.07
	Api/Baso	7.07±0.19	0.77±0.16^**	0.85±0.16^**	2.48±0.04^**	1.50±0.11^**	7.00±0.18
4hr	Api (nmol)	5.04±0.61	0.89±0.25^**	2.16±0.35^**	3.62±0.94	2.30±0.22^**	4.61±0.72
	Baso (nmol)	0.62±0.08	0.97±0.21^**	2.05±0.17^**	1.15±0.20 *	1.14±0.09^**	0.59±0.10
	Api/Baso	8.13±0.27	0.92±0.16^**	1.05±0.23^**	3.15±0.10^**	2.02±0.11^**	7.81±0.06

Significant difference from control *𝑃<.05, ^**𝑃<.01.