Functional Characterization of N-Terminally GFP-Tagged GLP-1 Receptor
Figure 2
Expression analysis of recombinant GFP-GLP-1 receptor from CHO cells. (a) CHO cells expressing GFP-GLP-1 receptor are viewed under standard light conditions or under argon-ion laser light source (488 nm) 24-hour posttransfection (merge pictures). (b) Western blot analysis with the anti-GFP primary polyclonal mouse IgG antibodies. Lane 1: membranes with the GFP-GLP-1 receptor; lane 2: membranes with the GFP; lane 3: membranes CHO. (c) Western blot analysis with the anti-GLP-1R primary polyclonal rabbit IgG antibodies. Lane 1: membranes CHO; lane 2: membranes with the GFP-GLP-1 receptor; lane 3: membranes with the GLP-1 receptor. The amount of protein loaded in each well in SDS-PAGE was 50 g.
