Functional Characterization of N-Terminally GFP-Tagged GLP-1 Receptor

Table 2

Effect of GLP-1(7-36)amide on insulin release from Rin m5F cells transiently expressing human GFP-GLP-1 receptor. Each bar with the indicated standard deviation represents the mean value of five independent experiments done in duplicate. ANOVA: F(3, 16) =𝟐𝟗𝟏.𝟔; P < .0001. Tukey-Kramer multiple comparison test was used to reveal significant differences between groups of interest.

Condition	Insulin release (𝜇U/50×10^4cells/h)
Basal (no addition of GLP-1(7-36)amide)	21.5±1.7
+ GLP-1(7-36)amide	69.9±5.4^∗
+ GFP-GLP-1 receptor	26.9±3.2
+ GFP-GLP-1 receptor + GLP-1(7-36)amide	388.5±45.6^∗∗

^∗𝑃<.05 versus basal (no addition).
^∗∗𝑃<.001 versus + GFP-GLP-1 receptor.