Identification of IbeR as a Stationary-Phase Regulator in Meningitic Escherichia coli K1 that Carries a Loss-of-Function Mutation in rpoS

Table 1

E. coli K1 (meningitic) or K12 (nonpathogenic) strains and plasmids used in this study.

Strain or plasmid	Characteristics	Reference(s)
Strains
RS218	O18:K1:H7 (CSF)	[6–9]
E44	RS218, Rif^r	[6–9]
DH5𝛼 (𝜆pir)	K 12 strain	[6, 7]
SM10 (𝜆pir)	K 12 strain	[6, 7]
MC4100	K 12 strain	[5]
RH90	K 12 strain, rpoS deletion mutant	[5]
BR2	ibeR deletion mutant of E44	This study
TNA44	tnaA deletion mutant of E44	This study
Plasmids
pCVD442	Amp^r, oiRr6K, sacB, mobRP4	[10, 11]
pCBR2	pCVD442 carrying an ibeR deletion, Amp^r	This study
pGEM-T	Amp^r, lacZ	Promega
pCTNA2	pCVD442 carrying a tnaA deletion, Amp^r	This study
pGTNA	pGEM-T carrying a 3.7 kb fragment containing tnaA gene	This study
pStyABB	containing the gene of monooxygenase for indole assay, Amp^r	[12]
pWKS30	Amp^r, lacZ	[6]
pWKS1030	pWKS30 carrying an 18 Kb ibeR locus	[6]

Amp^r, ampicillin resistant; lacZ, a partial gene coding for the N-terminal fragment of 𝛽-galactosidase; Kan^r, kanamycin resistant; Rif^r, rifampin resistant.