Identification of IbeR as a Stationary-Phase Regulator in Meningitic Escherichia coli K1 that Carries a Loss-of-Function Mutation in rpoS

Table 2

Oligonucleotides used for cloning, sequencing, and making the deletion mutants of ibeR and tnaA genes.

Primers	Sequences of primers	Retained amino acids
primers for ibeR deletion		Total 56 residues
IbeR-S1 (S = SalI)	5′-GATGTCGACGGGCTTTTCGGCGTCA-3′	52 N-terminal residues
IbeR-B1 (B = BamHI)	5′–CGGGATCCAGTGGCGAGGGTCACA-3′	(MDIIIMNKES...)
IbeR-B2 (B = BamHI)	5′-CAGGATCCAAATGTTGAGCATGCAG-3′	4 C-terminal residues
IbeR-X2 (X = XbaI)	5′-CGTCTAGATAAGGGCTAAACATATCG-3′	(...GSKC)
primers for tnaA deletion		Total 56 residues
TN-S1 (S = SalI)	5′-GGGTCGACCAGAGATCTGGCCGGAAT T-3′	21 N-terminal residues
TN-B1 (B = BamHI)	5′-ACGGATCCAATAACACGAATGCGGAACGGTTC-3′	(MKDYVMENFK...)
TN-B2 (B = BamHI)	5′-TTAGATCTTTTAAACATGTGAAAGAGAACGCG-3′	35 C-terminal residues
TN-X2 (X = XbaI)	5′-CCTCTAGATTAGCCAAATTTAGGTAACAC G-3′	(...RHFTAKLKEV)