BioMed Research International / 2009 / Article / Tab 2

Research Article

High Specificity of Quantitative Methylation-Specific PCR Analysis for MGMT Promoter Hypermethylation Detection in Gliomas

Table 2

MGMT promoter methylation status in primary tumour and recurrences by quantitative methylation-specific PCR (QMSP) and Methylation-Specific PCR (MSP).

PatientHystological* typeMonths§Sample#QMSPQMSPMSP
MedianIQR

G3GB0FFPE28.4113.28–47.82pospos
GB28FFPE12.811.07–16.00pospos
GB29FFPE209.37101.70–226.68pospos

G8GB0FFPE00-0negneg
GB10FFPE00-0negneg

G24GB0FFPE00-0negneg
GB9SF5.984.37–7.60pospos

*GB: Glioblastoma. §number of months from first surgery. #FFPE: Formalin fixed paraffin-embedded sample; SF: Snap-frozen pecimen. IQR: Interquartile range; pos: Methylation levels for QMSP exceed the cutoff point of 0.35, a band is seen in gel electrophoresis for MSP; neg: Methylation levels for QMSP are below the cutoff point of 0.35, no band is seen in gel electrophoresis for MSP.

We are committed to sharing findings related to COVID-19 as quickly and safely as possible. Any author submitting a COVID-19 paper should notify us at help@hindawi.com to ensure their research is fast-tracked and made available on a preprint server as soon as possible. We will be providing unlimited waivers of publication charges for accepted articles related to COVID-19. Sign up here as a reviewer to help fast-track new submissions.