Molecular Characterization of the Ghrelin and Ghrelin Receptor Genes and Effects on Fat Deposition in Chicken and Duck

Table 1

Chicken and duck samples used in this study.

No.	Species	Population(1)	Samples	Blood/live tissues	Purpose
POP1	Chicken	XH	6(3♂;3♀)	Blood for DNA	GHRL and GHSR gene variation
POP2	Chicken	WRR	6(3♂;3♀)
POP3	Chicken	TS	6(3♂;3♀)
POP4	Chicken	LH	6(3♂;3♀)
POP5	Chicken	XH&WRR	373(200♂;173♀)	Blood for DNA	Association analysis
POP6	Chicken	LY	6(3♂;3♀)	18 tissues (2)	Real time PCR
POP7	Chicken	HY	6(3♂;3♀)
POP8	Chicken	LY	2♀	Subcutaneous and abdominal adipose tissues	Cell culture
POP9	Duck	SW	1♂	Blood for DNA and proventriculus tissue	GHRL gene sequence cloning
POP10	Duck	SW	6(3♂;3♀)	Blood for DNA	GHRL and GHSR gene variation
POP11	Duck	PK	6(3♂;3♀)
POP12	Duck	PT	6(3♂;3♀)
POP13	Duck	LK	6(3♂;3♀)
POP14	Duck	SW	100(52♂;48♀)	Blood for DNA	Association analysis
POP15	Duck	SW	6(3♂;3♀)	18 tissues	Real time PCR
POP16	Duck	PT	6(3♂;3♀)
POP17	Duck	SW	2♀	Subcutaneous and abdominal adipose tissues	Cell culture

(1)XH = Xinghua chicken, WRR = White Recessive Rock, TS = Taihe Silkies, LH = Leghorn, XH&WRR = an F2 population crossed by XH and WRR, LY = Lingnan Yellow, HY = Huiyang Beard, SW = Sanshui White duck, PK = Peking duck, PT = Partridge duck, and LK = Lake duck.
(2) The 18 tissues used were pituitary, cerebrum, lung, abdominal fat, liver, testis, proventriculus, ovary, subcutaneous fat, spleen, kidney, oviduct, leg muscle, uropygial gland, hypothalamus, glandularis, small intestine, cerebellum, heart and breast muscle.