Research Article
Molecular Characterization of the Ghrelin and Ghrelin Receptor Genes and Effects on Fat Deposition in Chicken and Duck
Table 2
Descriptions of the 33 primers used in this study.
| Gene | Primer | Forward/reverse | Sequence ( to ) | Annealing temp () | Purpose |
| cGHRL | PM1 | PM1F | tgctgaaggaccgaaaacaaa | 56 | SNP identification | PM1R | gccttgacagatgccttagtg | PM2 | PM2F | aagaaagctggtaactgcactag | 55 | SNP identification | PM2R | ggtgggctggtggagtta | PM3 | PM3F | tgcgttctgctactctttttcat | 63 | SNP identification | PM3R | gggccaaggaggagtgtct | PM4 | PM4F | cggcagacactcctcctt | 57 | SNP identification | PM4R | gccatccttccaactgtgtatat | PM5 | PM5F | tatgcgttctgctactcttt | 58 | Genotyping by sequencing | PM5R | tggaagcgatcactatacc | PM6 | PM6F | catacagcaacaaaaggatac | 63 | Real time PCR | PM6R | tgtggttgtccttcagct |
| cGHSR | PM7 | PM7F | gtgggtcagggcatcaaactc | 58 | SNP identification and PCR-RFLP | PM7R | tcgagggctgctgaattttatg | PM8 | PM8F | ggctcttcctttttggtttgtct | 60 | SNP identification and PCR-RFLP | PM8R | tcgccctctctctgattcacct | PM9 | PM9F | tgatgccactggtctgagaat | 58 | Genotyping by PCR-RFLP | PM9R | tatccagctgcccatgtaaat | PM10 | PM10F | tgggcgtcgagcatgagaat | 63 | Real time PCR | PM10R | ccacgactagcatcttcacag |
| Chicken -actin | PM11 | PM11F | ccccaaagccaacagagaga | 63 | Real time PCR | PM11R | ggtggtgaagctgtagcctctc |
| dGHRL | PM12 | PM12F | Genomic Walking Kit | According to the kit | Genomic Walking | PM12R1 | ttccatcagttctagacgagt | PM12R2 | tgtttgtccatactcttgatact | PM12R3 | gcccctgctttatctgtatc | PM13 | PM13F | ccgcctggtgaagaaaac | 56 | RT-PCR | PM13R | aagcctacacatccacctgcaat | PM14 | PM14F | gaggcaagctgaaggacaaccaca | 54 | RACE | PM14R | RACE Kit | PM15 | PM15F | gctggttttcccgtgtaattc | 56 | RT-PCR | PM15R | tgtttgtccatactcttgatact | PM16 | PM16F | gctggttttcccgtgtaattc | 54 | Intron 1 amplification | PM16R | tgtttgtccatactcttgatact | PM17 | PM17F | tggtttggctggctctagt | 56 | Intron 2 amplification | PM7R | PM12R3 | PM18 | PM18F | aaagcaggggcagaagat | 57 | Intron 3 amplification | PM18R | PM12R2 | PM19 | PM19F | agggtcctggtccaaaaat | 54 | Intron 4 amplification | PM19R | PM12R1 | PM20 | PM20F | cgcatggtagccttcacacac | 56 | SNP identification and PCR-RFLP | PM20R | agccgatgggttagcagagag | PM21 | PM21F | tcggctccatcagttgcagttat | 56 | SNP identification | PM21R | cggcgatgtatttttgctgttg | PM22 | PM22F | tccccacgacagagtagtttgag | 56 | SNP identification and PCR-RFLP | PM22R | gccttcccctgcttcctaa | PM23 | PM23F | atagcagcattttagaagtga | 54 | SNP identification | PM23R | ttgcctcagcttttcact | PM24 | PM24F | cgtgtaattcctctctgctaa | 63 | Real time PCR | PM24R | cgatgtatttttgctgttgtt | dGHSR | PM25 | PM25F | cccctgagcaccaacgagt | 56 | Intron 1 amplification | PM25R | ggcaaccagcagagtatga | PM26 | PM26F | Genomic Walking Kit | According to the kit | Genomic Walking | PM26R1 | cggaccgatgttctttctcttt | PM26R2 | gacgggcaggaaaaagaagac | PM26R3 | ggcccagaggatgaggatga | PM27 | PM27F | tggcgttctccgacctgctcat | 57 | SNP identification | PM27R | acacagaccctcagaaacac | PM28 | PM28F | cgggtggcttaaataacaacag | 56 | SNP identification | PM28R | tgccctttttcctcagtttctc | PM29 | PM29F | cggaccataattaaacacctgaa | 56 | SNP identification | PM29R | caggtagaagaggacaaaggaca | PM30 | PM30F | tggcgttctccgacctgctcat | 56 | Genotyping by PCR-RFLP | PM30R | acacagaccctcagaaacac | PM31 | PM31F | gcaggttgtttagatatggct | 56 | Genotyping by PCR-RFLP | PM31R | cgtgaaaaggcaaccagcagag | PM32 | PM32F | cccctgagcaccaacgagt | 63 | Real time PCR | PM32R | ccaccacaactaacattttcaca |
| Duck -actin | PM33 | PM33F | acgccaacacggtgctg | 63 | Real time PCR | PM33R | gggtccggattcatcatactc |
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