Molecular Characterization of the Ghrelin and Ghrelin Receptor Genes and Effects on Fat Deposition in Chicken and Duck

Table 2

Descriptions of the 33 primers used in this study.

Gene	Primer	Forward/reverse	Sequence (5^′ to 3^′)	Annealing temp (^∘C)	Purpose
cGHRL	PM1	PM1F	tgctgaaggaccgaaaacaaa	56	SNP identification
		PM1R	gccttgacagatgccttagtg
	PM2	PM2F	aagaaagctggtaactgcactag	55	SNP identification
		PM2R	ggtgggctggtggagtta
	PM3	PM3F	tgcgttctgctactctttttcat	63	SNP identification
		PM3R	gggccaaggaggagtgtct
	PM4	PM4F	cggcagacactcctcctt	57	SNP identification
		PM4R	gccatccttccaactgtgtatat
	PM5	PM5F	tatgcgttctgctactcttt	58	Genotyping by sequencing
		PM5R	tggaagcgatcactatacc
	PM6	PM6F	catacagcaacaaaaggatac	63	Real time PCR
		PM6R	tgtggttgtccttcagct
cGHSR	PM7	PM7F	gtgggtcagggcatcaaactc	58	SNP identification and PCR-RFLP
		PM7R	tcgagggctgctgaattttatg
	PM8	PM8F	ggctcttcctttttggtttgtct	60	SNP identification and PCR-RFLP
		PM8R	tcgccctctctctgattcacct
	PM9	PM9F	tgatgccactggtctgagaat	58	Genotyping by PCR-RFLP
		PM9R	tatccagctgcccatgtaaat
	PM10	PM10F	tgggcgtcgagcatgagaat	63	Real time PCR
		PM10R	ccacgactagcatcttcacag
Chicken 𝛽-actin	PM11	PM11F	ccccaaagccaacagagaga	63	Real time PCR
		PM11R	ggtggtgaagctgtagcctctc
dGHRL	PM12	PM12F	Genomic Walking Kit	According to the kit	Genomic Walking
		PM12R1	ttccatcagttctagacgagt
		PM12R2	tgtttgtccatactcttgatact
		PM12R3	gcccctgctttatctgtatc
	PM13	PM13F	ccgcctggtgaagaaaac	56	RT-PCR
		PM13R	aagcctacacatccacctgcaat
	PM14	PM14F	gaggcaagctgaaggacaaccaca	54	3^′ RACE
		PM14R	3^′ RACE Kit
	PM15	PM15F	gctggttttcccgtgtaattc	56	RT-PCR
		PM15R	tgtttgtccatactcttgatact
	PM16	PM16F	gctggttttcccgtgtaattc	54	Intron 1 amplification
		PM16R	tgtttgtccatactcttgatact
	PM17	PM17F	tggtttggctggctctagt	56	Intron 2 amplification
		PM7R	PM12R3
	PM18	PM18F	aaagcaggggcagaagat	57	Intron 3 amplification
		PM18R	PM12R2
	PM19	PM19F	agggtcctggtccaaaaat	54	Intron 4 amplification
		PM19R	PM12R1
	PM20	PM20F	cgcatggtagccttcacacac	56	SNP identification and PCR-RFLP
		PM20R	agccgatgggttagcagagag
	PM21	PM21F	tcggctccatcagttgcagttat	56	SNP identification
		PM21R	cggcgatgtatttttgctgttg
	PM22	PM22F	tccccacgacagagtagtttgag	56	SNP identification and PCR-RFLP
		PM22R	gccttcccctgcttcctaa
	PM23	PM23F	atagcagcattttagaagtga	54	SNP identification
		PM23R	ttgcctcagcttttcact
	PM24	PM24F	cgtgtaattcctctctgctaa	63	Real time PCR
		PM24R	cgatgtatttttgctgttgtt
dGHSR	PM25	PM25F	cccctgagcaccaacgagt	56	Intron 1 amplification
		PM25R	ggcaaccagcagagtatga
	PM26	PM26F	Genomic Walking Kit	According to the kit	Genomic Walking
		PM26R1	cggaccgatgttctttctcttt
		PM26R2	gacgggcaggaaaaagaagac
		PM26R3	ggcccagaggatgaggatga
	PM27	PM27F	tggcgttctccgacctgctcat	57	SNP identification
		PM27R	acacagaccctcagaaacac
	PM28	PM28F	cgggtggcttaaataacaacag	56	SNP identification
		PM28R	tgccctttttcctcagtttctc
	PM29	PM29F	cggaccataattaaacacctgaa	56	SNP identification
		PM29R	caggtagaagaggacaaaggaca
	PM30	PM30F	tggcgttctccgacctgctcat	56	Genotyping by PCR-RFLP
		PM30R	acacagaccctcagaaacac
	PM31	PM31F	gcaggttgtttagatatggct	56	Genotyping by PCR-RFLP
		PM31R	cgtgaaaaggcaaccagcagag
	PM32	PM32F	cccctgagcaccaacgagt	63	Real time PCR
		PM32R	ccaccacaactaacattttcaca
Duck 𝛽-actin	PM33	PM33F	acgccaacacggtgctg	63	Real time PCR
		PM33R	gggtccggattcatcatactc