Molecular Characterization of the Ghrelin and Ghrelin Receptor Genes and Effects on Fat Deposition in Chicken and Duck

Table 3

SNPs identified in the cGHRL, cGHSR, dGHRL, and dGHSR genes.

Gene	No.	SNP1	Region	Note (2,3,4)	No.	SNP	Region	Note (2,3,4)
cGHRL	1	C-495T	5^′ flanking	19369777(2)Dde I (3)	11	A-2220C	5^′ flanking	19371502
	2	C-517T	5^′ flanking	19369799	12	A-2246G	5^′ flanking	19371528 Tas I
	3	C-873G	5^′ flanking	19370155	13	A-2355C	5^′ flanking	19371637
	4	T-1038C	5^′ flanking	19370320	14	C-2399del	5^′ flanking	19371681
	5	C-1253T	5^′ flanking	19370535 Hin1 II	15	G-2861T	5^′ flanking	19372143
	6	T-1562G	5^′ flanking	19370844	16	G-3264C	5^′ flanking	19372546
	7	A-1589G	5^′ flanking	19370871	17	C-3290T	5^′ flanking	19372572 Tai I
	8	G-1950A	5^′ flanking	19371232	18	G-3321T	5^′ flanking	19372603
	9	C-2030T	5^′ flanking	19371312	19	T-3324C	5^′ flanking	19372606
	10	C-2046G	5^′ flanking	19371329
cGHSR	1	A-1907G	5^′ flanking	18787994	5	T-1288C	5^′flanking	18789055
	2	C-1896A	5^′ flanking	18788005 Tat I	6	A-1022C	5^′ flanking	18789321 Tail I
	3	C-1608T	5^′ flanking	18788293	7	T-892C	5′ flanking	18789762 Bsa JI
	4	C-1459T	5^′ flanking	18788442 Hin1 II	8	G-834C	5^′ flanking	18789820 Hin6 I
dGHRL	1	G221A	5^′ flanking		23	T1128C	Exon 2
	2	T256C	5^′ flanking		24	T1145C	Exon 2	G5 Syn(4), Csp6 I
	3	T263C	5^′ flanking		25	C1179G	Exon 2	R17G, Msp I
	4	G273T	5′ flanking		26	A1244T	Intron 2
	5	A276C	5^′ flanking		27	T1278C	Intron 2
	6	A303G	5^′ flanking		28	G1390A	Intron 2
	7	A342C	5^′ flanking		29	A1756G	Exon 3	T53A, Hin 6 I
	8	T401C	5^′ flanking	Csp6 I	30	G1761A	Exon 3	E54 Syn
	9	C405T	5^′ flanking		31	G1774A/C	Exon 3	A59P/T
	10	A532C	5^′ flanking		32	A1789G	Exon 3	T64A
	11	A628G	5^′ flanking		33	C1803T	Exon 3	N68 Syn
	12	C638T	5^′ flanking		34	A1807G	Exon 3	G70S
	13	A645G	5^′ flanking		35	A1943C	Intron 3	HpyCH4IV
	14	C686G	5^′ flanking		36	C2070T	Intron 3
	15	C691A	5^′ flanking		37	T2115C	Intron 3	TailI
	16	A736T	5^′ flanking		38	C2179T	Intron 3
	17	C778A	5^′ flanking		39	A2324G	Exon 4	E89 Syn
	18	C1028A	Intron 1		40	G2345A	Exon 4	T96 Syn
	19	A1070G	Intron 1		41	A2351G	Exon 4	E98 Syn
	20	A1108G	Exon 2		42	G2409C	Intron 4
	21	A1118G	Exon 2		43	T2509C	Intron 4
	22	A1117G	Exon 2
dGHSR	1	C320T	Exon 1	A Syn, Msp 𝐼^(4)	25	C2815A	Intron
	2	T359C	Exon 1	N Syn	26	C2843T	Intron
	3	G398C	Exon 1	T Syn, Bsa JI	27	C2861T	Intron
	4	T404C	Exon 1	Y Syn, Csp6 I	28	G2863C	Intron
	5	G548A	Exon 1	T Syn	29	C2883T	Intron
	6	C779T	Intron		30	T2906C	Intron
	7	T1364A	Intron		31	C2939A	Intron
	8	C1437T	Intron		32	T2958A	Intron
	9	T1456C	Intron		33	G2993A	Intron
	10	T1469C	Intron		34	G3001A	Intron
dGHSR	11	C1478T	Intron		35	A3304G	Intron
	12	A2393T	Intron		36	A3037G	Intron
	13	C2430T	Intron		37	T3050G	Intron
	14	A2431G	Intron		38	G3080A	Intron
	15	T2578C	Intron		39	T3208A	Intron	Taa I
	16	T2591A	Intron	Tail I	40	C3329T	Intron	Taa I
	17	T2619A	Intron		41	T3340A	Intron
	18	C2657T	Intron		42	G3362A	Intron
	19	C2681T	Intron		43	G3363A	Intron
	20	A2682G	Intron		44	T3380C	Intron
	21	G2746A	Intron		45	C3424T	Intron	Csp6 I
	22	T2792C	Intron		46	A3427G	Intron
	23	G2794T	Intron		47	A3451G	Intron
	24	G2803A	Intron		48	C3543T	Intron

(1)The first nucleotide of start codon was marked as +1, and the next upstream nucleotide was −1. SNP position was determined based on reported sequences of GenBank EF613552 (dGHRL gene) and FJ194548 (dGHSR gene), respectively. (2)SNP position was determined according to the released chicken genomic sequence (http://genome.ucsc.edu/).(3)Restriction enzyme was used for PCR-RFLP. (4)SNP caused a codon and amino acid change.