BioMed Research International / 2009 / Article / Tab 3

Research Article

Molecular Characterization of the Ghrelin and Ghrelin Receptor Genes and Effects on Fat Deposition in Chicken and Duck

Table 3

SNPs identified in the cGHRL, cGHSR, dGHRL, and dGHSR genes.

GeneNo.SNP RegionNote No.SNPRegionNote

cGHRL1C-495T flanking19369777 Dde I 11A-2220C flanking19371502
2C-517T flanking1936979912A-2246G flanking19371528 Tas I
3C-873G flanking1937015513A-2355C flanking19371637
4T-1038C flanking1937032014C-2399del flanking19371681
5C-1253T flanking19370535 Hin1 II 15G-2861T flanking19372143
6T-1562G flanking1937084416G-3264C flanking19372546
7A-1589G flanking1937087117C-3290T flanking19372572 Tai I
8G-1950A flanking1937123218G-3321T flanking19372603
9C-2030T flanking1937131219T-3324C flanking19372606
10C-2046G flanking19371329

cGHSR1A-1907G flanking187879945T-1288C flanking18789055
2C-1896A flanking18788005 Tat I 6A-1022C flanking18789321 Tail I
3C-1608T flanking187882937T-892C5′ flanking18789762 Bsa JI
4C-1459T flanking18788442 Hin1 II 8G-834C flanking18789820 Hin6 I

dGHRL1G221A flanking23T1128CExon 2
2T256C flanking24T1145CExon 2G5 Syn , Csp6 I
3T263C flanking25C1179GExon 2R17G, Msp I
4G273T5′ flanking26A1244TIntron 2
5A276C flanking27T1278CIntron 2
6A303G flanking28G1390AIntron 2
7A342C flanking29A1756GExon 3T53A, Hin 6 I
8T401C flankingCsp6 I30G1761AExon 3E54 Syn
9C405T flanking31G1774A/CExon 3A59P/T
10A532C flanking32A1789GExon 3T64A
11A628G flanking33C1803TExon 3N68 Syn
12C638T flanking34A1807GExon 3G70S
13A645G flanking35A1943CIntron 3HpyCH4IV
14C686G flanking36C2070TIntron 3
15C691A flanking37T2115CIntron 3TailI
16A736T flanking38C2179TIntron 3
17C778A flanking39A2324GExon 4E89 Syn
18C1028AIntron 140G2345AExon 4T96 Syn
19A1070GIntron 141A2351GExon 4E98 Syn
20A1108GExon 242G2409CIntron 4
21A1118GExon 243T2509CIntron 4
22A1117GExon 2

dGHSR1C320TExon 1A Syn, Msp 25C2815AIntron
2T359CExon 1N Syn26C2843TIntron
3G398CExon 1T Syn, Bsa JI 27C2861TIntron
4T404CExon 1Y Syn, Csp6 I 28G2863CIntron
5G548AExon 1T Syn29C2883TIntron
6C779TIntron30T2906CIntron
7T1364AIntron31C2939AIntron
8C1437TIntron32T2958AIntron
9T1456CIntron33G2993AIntron
10T1469CIntron34G3001AIntron
dGHSR11C1478TIntron35A3304GIntron
12A2393TIntron36A3037GIntron
13C2430TIntron37T3050GIntron
14A2431GIntron38G3080AIntron
15T2578CIntron39T3208AIntronTaa I
16T2591AIntronTail I40C3329TIntronTaa I
17T2619AIntron41T3340AIntron
18C2657TIntron42G3362AIntron
19C2681TIntron43G3363AIntron
20A2682GIntron44T3380CIntron
21G2746AIntron45C3424TIntronCsp6 I
22T2792CIntron46A3427GIntron
23G2794TIntron47A3451GIntron
24G2803AIntron48C3543TIntron

The first nucleotide of start codon was marked as +1, and the next upstream nucleotide was 1. SNP position was determined based on reported sequences of GenBank EF613552 (dGHRL gene) and FJ194548 (dGHSR gene), respectively. SNP position was determined according to the released chicken genomic sequence (http://genome.ucsc.edu/). Restriction enzyme was used for PCR-RFLP. SNP caused a codon and amino acid change.

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