A Novel Method for High-Level Production of TEV Protease by Superfolder GFP Tag
Figure 3
SDS-PAGE and time course analysis of catalytic activity on MBP-EGFP of sfGFP-TEV-His6 and TEV-His6. (a) One representative of SDS-PAGE analysis. 100 g MBP-EGFP was incubated with 2 g sfGFP-TEV-His6 Nd2. Lane 1 is the protein marker. Lanes 2–11 represent different incubation time (0, 5, 10, 20, 40, 60, 90, 120, 180, 240 minutes, resp.). (b) The time course curve analysis. The time course curve was plotted according to the quantitative analysis of the SDS-PAGE by Bandscan 4.30. Each data point was the average of three independent tests. (c) Bar representation based on time course curve. The black bars represent the percentage of the digested MBP-EGFP after incubation for 60 minutes. The striped bars represent the percentage after incubation for 240 minutes. The ranking of the bar is based on the sorting result of cleavage efficiency after incubation for 60 minutes. The height of the bar is the average of three independent tests with standard error on top of the bar.
